Ruobin Wei
Laboratory >>>
Applied Biotechnology Coursework
In spring 2018, I took the Applied Biotechnology course at San Diego Miramar College. This class provided me with approximately 100 hours of hands-on practice in biotechnology-related experiments such as PCR, gel electrophoresis, column chromatography, DNA extraction, protein purification and quantitation, bacterial transformation, cell transfection, ELISA, Western blotting, SDS-PAGE and staining, and flow cytometry. This class also helped me become familiar with Good Manufacturing Practice (GMP), as well as following and developing a standard operating procedure (SOP). I really enjoyed learning about these biotechnology skills, and I hope to apply my learnings to future jobs or academic settings in the field of biology and nutritional sciences. The pictures show some of the experiments I completed during the course.
Red Fluorescent Protein Purification
with Column Chromatography
Plasmid DNA Restriction Digestion
BCA Assay
Mammalian Cell Culturing
Lab Assistant
In the school year of 2015-2016, I worked as a lab assistant under Dr. Craig Miller at UC Berkeley. The lab studied genetics and ecology issues using three spine stickleback fish as the subject. My duty as a lab assistant was to feed the fish using designated food and tools, adjust the amount and type of food given based on the behaviors and growth of the fish, and maintaining the general well-functioning of the fish room, such as taking out and labeling dead fish, and keeping the working area clean.
Chemistry & Biology Lab Skills
Throughout my first two years in college, I have accumulated skills in conducting chemistry and biology experiments in a classroom setting. The picture on the right shows me doing a chemistry experiment. I have attached examples of my chemistry and biology lab reports in the following links.